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clone db105  (Miltenyi Biotec)


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    Miltenyi Biotec clone db105
    Clone Db105, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/clone db105/product/Miltenyi Biotec
    Average 95 stars, based on 31 article reviews
    clone db105 - by Bioz Stars, 2026-03
    95/100 stars

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    Primer sequence list

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: SOX2OT variant 7 contributes to the synergistic interaction between EGCG and Doxorubicin to kill osteosarcoma via autophagy and stemness inhibition

    doi: 10.1186/s13046-018-0689-3

    Figure Lengend Snippet: Primer sequence list

    Article Snippet: Mouse anti-Human CD133/1 (Clone: AC133)-PE and mouse anti-human CD44 (Clone: DB105)-FITC (Miltenyi Biotec, USA) were used.

    Techniques: Sequencing

    EGCG reduced the expression of CSC markers of osteosarcoma stem cell. a OSCs obtained from osteosarcoma cell line U2OS by CD133+/CD44+ immunomagnetic beads double positive screening and the isolated CD133+/CD44+ OSCs were identified by flow cytometry analysis. U2OS parental cells were set as Control (Con). b The relative mRNA levels of CSC markers Nanog, Sox2 and OCT4 in different bead-sorted cells were checked by qRT-PCR. * p < 0.05 vs. parental cells, ** p < 0.01 vs. parental cells, # p < 0.05 vs. CD133+ cells. c qRT-PCR was performed to detect the SOX2OT V7 in different bead-sorted cells. * p < 0.05 vs. parental cells (Con). d Relative mRNA levels of CSC markers Nanog, Sox2, OCT4, c-Myc and ABCG2 in OSCs treated with or without EGCG were detected by qRT-PCR. * p < 0.05 vs. parental cells (Con), ** p < 0.01 vs. parental cells (Con), # p < 0.05 vs. OSC. Results shown are representative of three independent experiments and error bars indicate SD. e Expression of CSC markers Nanog, Sox2, OCT4, c-Myc and ABCG2 in OSCs treated with or without EGCG were detected by western blots

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: SOX2OT variant 7 contributes to the synergistic interaction between EGCG and Doxorubicin to kill osteosarcoma via autophagy and stemness inhibition

    doi: 10.1186/s13046-018-0689-3

    Figure Lengend Snippet: EGCG reduced the expression of CSC markers of osteosarcoma stem cell. a OSCs obtained from osteosarcoma cell line U2OS by CD133+/CD44+ immunomagnetic beads double positive screening and the isolated CD133+/CD44+ OSCs were identified by flow cytometry analysis. U2OS parental cells were set as Control (Con). b The relative mRNA levels of CSC markers Nanog, Sox2 and OCT4 in different bead-sorted cells were checked by qRT-PCR. * p < 0.05 vs. parental cells, ** p < 0.01 vs. parental cells, # p < 0.05 vs. CD133+ cells. c qRT-PCR was performed to detect the SOX2OT V7 in different bead-sorted cells. * p < 0.05 vs. parental cells (Con). d Relative mRNA levels of CSC markers Nanog, Sox2, OCT4, c-Myc and ABCG2 in OSCs treated with or without EGCG were detected by qRT-PCR. * p < 0.05 vs. parental cells (Con), ** p < 0.01 vs. parental cells (Con), # p < 0.05 vs. OSC. Results shown are representative of three independent experiments and error bars indicate SD. e Expression of CSC markers Nanog, Sox2, OCT4, c-Myc and ABCG2 in OSCs treated with or without EGCG were detected by western blots

    Article Snippet: Mouse anti-Human CD133/1 (Clone: AC133)-PE and mouse anti-human CD44 (Clone: DB105)-FITC (Miltenyi Biotec, USA) were used.

    Techniques: Expressing, Isolation, Flow Cytometry, Control, Quantitative RT-PCR, Western Blot